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本研究组已证实，部分去传入猫脊髓背角组织提取液中存在某种促神经元存活的神经营养活性物质。为了探讨针刺对该活性物质含量及其对神经元存活的影响，作者采用5只双侧备用根猫（切除双侧Ll～L5，L7～S2背根节，保留L6为备用根），每日针刺一侧L6背神经支配范围内的两组穴位（足三里与悬钟、伏兔与三阴交）之一，术后5天分别取双侧T12～S2背角组织，匀浆、离心，取上溶液进行电泳和细胞培养。经聚丙烯酸胺梯度电泳后行光密度扫描，发现针刺侧相对迁移率（RM）0.11蛋白带的吸收峰面积百分比（11．78±l．08％）较非针刺侧着（10．12±0.83％）明显增高（P＜0.05），表明针刺使RM0．11带蛋白质的含量增加。用本组建立的简易细胞钓蛋白带法将鸡胚背根节（DRG）的神经细胞悬液倒于电泳凝胶条上进行培养，发现凝胶条上RM0.11蛋白带所在区域内（RM0．10～0．13），有密集的神经细胞贴附。恒定面积由（1．02×104μm2／视野）内，针刺侧平均细胞数（19．09±2．12个）较非针刺侧者（8．64±1．12个）明显增多（P＜0．05）。聚丙烯酸胺梯度电泳测定分子量后显示RM0．11带蛋白质分子量约为130KD左右。上述结果表明，部分去传入猫背角组织中分子量约130KD左右的蛋白质具有营养DRG神经元的作用，针刺可能通过该类?
Our group has confirmed that some neurotrophic substances that promote the survival of neurons exist in some of the tissue extracts extracted from cat’s spinal cord. In order to investigate the effect of acupuncture on the content of the active substance and its effect on the survival of neurons, the authors used 5 bilateral standby root cats (resection of bilateral Ll ~ L5, L7 ~ S2 dorsal root ganglia, reserve L6 as spare root) Day acupuncture side L6 dorsal nerve within the scope of the two groups of points (Zusanli and hanging bell, V rabbit and Sanyinjiao) one of the five days after surgery were taken T12 ~ S2 dorsal horn organization, homogenate, centrifugation, Take the solution for electrophoresis and cell culture. After polyacrylamide gradient electrophoresis followed by optical density scanning, it was found that the percentage of peak absorption area of the 0.11 protein band (11.78 ± 1.08%) on the acupuncture side was significantly lower than that on the non-acupuncture side (10.12 ± 0.83%) (P <0.05), indicating that acupuncture increased the protein content of RM0.11. With this group established simple cell protein banding method will chick embryo dorsal root ganglia (DRG) nerve cell suspension was poured on the gel electrophoresis on the strip and found that RM0.11 protein band on the strip where the region (RM0 .10 ~ 0.13), there are dense nerve cells attached. The average number of cells on the acupuncture side (19.09 ± 2.12) was significantly higher than that on the non-acupuncture side (8.64 ± 1.12) in the constant area (1.02 × 104μm2 / visual field) <0.05). Polyacrylamide gradient electrophoresis after the determination of molecular weight showed RM0.11 with a protein molecular weight of about 130KD or so. The above results show that some of the proteins introduced into the dorsal horn of cats with a molecular weight of about 130KD have the function of vegetative DRG neurons.