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目的:为了证实月桂酸修饰的超氧化物歧化酶(AC-SOD)保护红细胞抗氧化溶血作用是否更优于未修饰的SOD.方法:先在人的红细胞悬液中分别加入AC-SOD(A组)、SOD(B组)、生理盐水(C组),于37℃温育20min后,洗掉未结合在膜上的酶,再于三组红细胞悬液中均加入黄嘌呤(X)和黄嘌呤氧化酶(XO)以产生超氧阴离子(O-2)造成溶血.各组再分别温育30min和1h,以红细胞加蒸馏水的全溶血试管作为对照,用分光光度法测光密度值,计算溶血率.结果:温育30min的溶血率,A,B,C三组分别为11.6±0.86,13.32±1.25和14.34±1.38,A组明显低于B组(P<0.05).B组与C组间无明显差异(P>0.05).温育1h的溶血率分别为16.36±0.48,23.54±1.49和27.66±2.30,A组非常显著地低于B组和C组(P<0.001).结论:月桂酸修饰的SOD保护红细胞抗氧化溶血作用明显优于未修饰的SOD.
OBJECTIVE: To verify if lauric acid-modified superoxide dismutase (AC-SOD) protects erythrocytes from anti-hemolysis better than unmodified SOD. Methods: AC-SOD (group A), SOD (group B) and normal saline (group C) were added to human erythrocyte suspension respectively and incubated at 37 ℃ for 20 min. , And xanthine (X) and xanthine oxidase (XO) were added to the three groups of erythrocyte suspensions to produce superoxide anion (O-2) to cause hemolysis. Each group were incubated for 30min and 1h respectively, and the hemolysis rate was calculated by using the hemolysate test tube of erythrocytes and distilled water as the control. The optical density was measured by spectrophotometry. Results: The rate of hemolysis after incubation for 30 min was 11.6 ± 0.86, 13.32 ± 1.25 and 14.34 ± 1.38 in group A, B and C respectively, which was significantly lower in group A than in group B P <0.05). There was no significant difference between group B and group C (P> 0.05). The hemolysis rates of 1h incubation were 16.36 ± 0.48, 23.54 ± 1.49 and 27.66 ± 2.30, respectively, which were significantly lower in group A than those in groups B and C (P <0.001 ). CONCLUSION: L-lauric acid modified SOD protects erythrocytes from being more resistant to hemolysis than unmodified SOD.