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用致病力为0的FOC-RA-5在黄瓜胚根长约1cm(浸胚根)和子叶展平时(灌根)分别进行2次诱导处理,以无菌水处理为对照,诱导处理后每隔24h取黄瓜叶片作为供试材料,对黄瓜叶片内苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)、脂氧合酶(LOX)、抗坏血酸过氧化物酶(APX)和硝酸还原酶(NR)活性进行测定,研究了黄瓜枯萎病菌弱毒菌株对黄瓜枯萎病诱导抗性机理。结果表明:叶片内LOX、PAL、PPO、POD活性先升高后降低,APX、NR活性先降低后升高,处理组的酶活性显著高于对照组的酶活性;黄瓜叶片内APX、LOX、PPO、POD和PAL活性与诱导后黄瓜植株的病情指数呈负相关,NR活性与诱导后黄瓜植株病情指数呈正相关。
Pathogenicity of 0 FOC-RA-5 in the cucumber radicle about 1cm (radicle radicle) and cotyledon flattening (irrigation) were induced twice, with sterile water as a control after induction treatment Cucumber leaves were taken as test material every 24h, and the contents of PAL, PPO, POD, LOX, Ascorbic acid peroxidase (APX) and nitrate reductase (NR) were measured to study the mechanism of induced resistance to Fusarium oxysporum f. The results showed that the activities of LOX, PAL, PPO and POD in leaves increased firstly and then decreased, APX and NR activities decreased first and then increased, the enzyme activities in treatment group were significantly higher than those in control group. The activities of APX, LOX, The activities of PPO, POD and PAL were negatively correlated with the disease index of cucumber plants. The NR activity was positively correlated with the disease index of cucumber plants.