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为研究3种不同的犬离体肺保存方式对支气管肺泡灌洗液中肺表面活性物质的影响。随机将30只Mongrel犬分成3组,为体外循环灌注组、间断压力灌注组和单纯低温保存组,每组10只。在保持机械通气的条件下,摘取犬双肺,3种不同方式保存离体肺,按时间点留取支气管肺泡灌洗液标本,BCA法测定总蛋白浓度,抗坏血酸法测定总磷脂的含量,薄层色谱分析测定二棕榈酸磷脂酰胆碱含量,酶联免疫吸附试验检测白介素(IL-1a/2/6/8)。随着犬肺离体时间的推移,各实验组离体肺支气管肺泡灌洗液中DPPC/PL的比值呈时间依赖性下降,各白介素/总蛋白比值呈时间依赖性升高。在同一时间点上,体外循环灌注组中DPPC/PL的比值最高,间断压力灌注组次之,单纯低温保存组最低;体外循环灌注组中各白介素/总蛋白的比值最低,间断压力灌注组次之,单纯低温保存组最高。采用体外循环机持续灌注犬离体肺,离体肺中可维持较高浓度的肺泡表面活性物质,维持肺泡表面张力,发挥肺保护作用,这可能与抑制炎症介质的释放有关。
To investigate the effects of three different types of isolated lung preservation in dogs on pulmonary surfactant in bronchoalveolar lavage fluid. 30 Mongrel dogs were randomly divided into 3 groups: cardiopulmonary bypass perfusion group, intermittent pressure perfusion group and hypothermia preservation group, with 10 in each group. Under the conditions of mechanical ventilation, dogs were harvested from the lungs, isolated lungs were preserved in 3 different ways, bronchoalveolar lavage fluid samples were taken at time points, total protein concentration was determined by BCA method, total phospholipid content was determined by ascorbic acid method, The content of phosphatidyl diphosterate was determined by thin-layer chromatography, and interleukin (IL-1a / 2/6/8) was detected by enzyme-linked immunosorbent assay. The DPPC / PL ratio in ex vivo pulmonary bronchoalveolar lavage fluid of each experimental group decreased in a time-dependent manner, and the interleukin / total protein ratio increased in a time-dependent manner with the time of in vitro ex vivo dog’s lung. At the same time point, the ratio of DPPC / PL in CPB group was the highest, followed by the intermittent pressure infusion group and lowest in the cryopreservation group. The ratio of interleukin / total protein was the lowest in CPB group, The highest, only low-temperature preservation group. Continuous perfusion of ex vivo isolated lungs with an extracorporeal circulation machine can maintain a high concentration of alveolar surfactant in ex vivo lungs, maintain the alveolar surface tension and exert lung protective effect, which may be related to the inhibition of the release of inflammatory mediators.