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目的探讨精氨酸-甘氨酸-天门冬氨酸(RGD)序列肽对TPTX低钙模型大鼠给予外源PTH后血钙水平的影响。方法雄性SD大鼠于麻醉下行TPTX术,建立低钙模型,16只造模成功按体重随机分为NS组和RGD组,分别予PTH1-34联合生理盐水和PTH1-34联合人工合成RGD序列肽(RGDY)持续输注6 h,监测血钙水平的动态变化。结果两组大鼠TPTX术后24 h血钙水平均降低至基线水平的70%左右,提示TPTX大鼠低钙血症模型建立。给予外源PTH后RGD组血钙水平恢复且显著低于NS组(F=16.465,P<0.05)。结论 RGDY阻抑了TPTX大鼠给予甲状旁腺激素后血钙的上升,RGD序列肽可能具有抑制破骨细胞骨吸收的功能,作为防治代谢性骨病的新型药物开发值得关注。
Objective To investigate the effect of arginine-glycine-aspartate (RGD) peptide on serum calcium levels after exogenous PTH administration in TPTX low calcium model rats. Methods Male SD rats underwent TPTX under anesthesia to establish a low-calcium model. Sixteen models were randomly divided into NS group and RGD group according to body weight. The rats were randomly divided into three groups: the combination of PTH1-34 with normal saline and PTH1-34, (RGDY) continuous infusion 6 h, to monitor the dynamic changes in serum calcium levels. Results The levels of serum calcium in 24 h after TPTX in both groups were reduced to about 70% of the baseline levels, suggesting that hypocalcemia model was established in TPTX rats. After administration of exogenous PTH, serum calcium level in RGD group was significantly lower than that in NS group (F = 16.465, P <0.05). Conclusion RGDY suppresses the increase of serum calcium in TPTX rats after administration of parathyroid hormone. RGD peptide may have the function of inhibiting the bone resorption of osteoclasts, which is worthy of attention as a new drug for the prevention and treatment of metabolic bone diseases.