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目的：探讨超氧化物歧化酶（ＳＯＤ）对肝癌细胞增殖的抑制作用。方法：利用逆转录病毒载体介导的基因转移技术，将人铜锌ＳＯＤ基因导入人肝癌细胞系ＨｅｐＧ２细胞内，获得高表达。检测其对ＨｅｐＧ２细胞生物学特性的影响。结果：与对照相比ＳＯＤ基因转染后的肝癌细胞生长受到抑制，细胞体积变大，分裂象减少，Ｓ期细胞比率减少，软琼脂集落形成率低，裸鼠成瘤瘤体小。结论：铜锌ＳＯＤ基因具有抑制ＨｅｐＧ２肝癌细胞增殖的作用。
Objective: To investigate the inhibitory effect of superoxide dismutase (SOD) on proliferation of hepatoma cells. METHODS: Human copper-zinc SOD gene was introduced into human hepatocellular carcinoma cell line HepG2 cells using a retroviral vector-mediated gene transfer technique to obtain high expression. Its effect on the biological characteristics of HepG2 cells was examined. RESULTS: Compared with the control, the growth of hepatoma cells transfected with SOD gene was inhibited, the cell volume became larger, the mitotic pattern decreased, the ratio of S phase cells decreased, the formation rate of soft agar colonies was low, and the tumor formation of nude mice was small. Conclusion: Copper-zinc SOD gene can inhibit the proliferation of HepG2 cells.