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目的 探讨不同照射方式对白细胞介素4 (IL-4)诱导的RAW264.7细胞向M2型巨噬细胞极化的影响.方法 以IL-4刺激RAW264.7细胞诱导其向M2型极化,在IL-4诱导细胞前、后分别进行不同剂量的照射,采用Westernblot检测不同刺激条件下RAW264.7细胞YM-1和Arg-1的表达水平,采用流式细胞术(FCM)检测不同刺激条件下RAW264.7细胞表面CD206和CD209的表达.结果 不同的照射方式均上调IL-4诱导的RAW264.7细胞YM-1和Arg-1的蛋白表达水平,在IL-4诱导细胞后进行照射比在IL-4诱导细胞前进行照射显著上调细胞YM-1和Arg-1的蛋白表达;照射能抑制IL-4诱导下细胞表面CD206和CD209的表达.结论 不同的照射方式促进IL-4诱导的RAW264.7细胞向M2型巨噬细胞极化,但抑制RAW264.7细胞的功能分子CD206和CD209的表达.“,”Objective To investigate the effect of radiotherapy on RAW264.7 cell polarization to M2 phenotype induced by interleukin-4 (IL-4).Methods RAW264.7 cells were induced to polarize to M2 by IL-4.CD206 and CD209 expressions induced by different radiation condition were detected by using flow cytomtery and protein expressions of Arginase1 (Arg-1)and YM-1 of M2 phenotypes were detected by Western blot,respectively.Results Radiotherapy promoted the increase of YM-1 and Arg-1 protein expressions induced by IL-4,but downregulated CD206 and CD209 expressions.Conclusion Radiotherapy can promote the macrophage polarization to M2 induced by IL-4 but downregulated functional molecular expression.