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目的探求调控骨髓间质干细胞中药的共性。方法硅胶柱层析龟板活性部位浸膏,用石油醚-乙酸乙酯作洗脱剂,梯度洗脱,得到16个样品;采用MTT法研究了它们对rMSCs的增殖调控作用,根据总离子图的相似保留时间和相应峰面积,获得促进rMSCs的增殖的指纹图;同样根据它们的总离子图的相似保留时间和相应峰面积也获得了抑制rMSCs增殖的指纹图;用中药龟板提取物有效部位指纹图谱预测中药汤剂“四物汤”和“栽培红厚壳果仁”共7个样品。结果活性实验结果显示Ts-2、Ts-3、Ts-11、Ts-12、Ts-16能促进rMSCs的增殖作用(P<0.05);Ts-4对rMSCs具有明显抑制作用,其他样品对rMSCs的增殖作用不显著(P>0.05),不具统计学意义;龟板活性部位指纹图谱预测其他中药活性正确率为86%。结论这种以生物活性为基础的指纹图谱具有科学性,为寻找相似活性的中药提供新思路。
Objective To explore the commonalities of traditional Chinese medicines regulating bone marrow mesenchymal stem cells. METHODS: The silica gel column chromatography was used to extract the active part of the tortoise shell, and 16 samples were obtained by gradient elution with petroleum ether-ethyl acetate as eluent. The proliferation regulation effect of rMSCs on the proliferation of rMSCs was studied by MTT method. Similar retention times and corresponding peak areas were used to obtain fingerprints that promote the proliferation of rMSCs; fingerprints that inhibit the proliferation of rMSCs were also obtained based on similar retention times and corresponding peak areas of their total ionograms; Maps predicted a total of 7 samples of traditional Chinese medicine decoction “Siwutang” and “cultivated red thick shell kernel”. Results The results of activity test showed that Ts-2, Ts-3, Ts-11, Ts-12, Ts-16 could promote the proliferation of rMSCs (P<0.05); Ts-4 had a significant inhibitory effect on rMSCs, and other samples were more sensitive to rMSCs. The proliferation was not significant (P>0.05), and it was not statistically significant. The active area fingerprint of the turtle shell predicted the activity rate of other Chinese herbs was 86%. Conclusion This biological activity-based fingerprint is scientific and provides new ideas for searching for similar active Chinese medicines.