通过黑荆树组织培养,探索基本培养基、生长调节物质、蔗糖含量、生长素种类和浓度不同对黑荆树嫩梢增殖、生根的影响。研究结果:采用春季培育42天的实生苗茎段作为外植体,在含有一定成分配比的培养基中,经7-20天,外植体长出1-3个芽梢。在低无机盐分的培养基中附加生长素NAA0.2-0.4mg/1,20-30天即可生根。研究指出:芽丛分化与细胞激动素,无机盐分含量,蔗糖浓度和生长素种类及浓度关系密切。 Through the tissue culture of Acacia scoparium, the effects of basic medium, growth regulators, sucrose content, auxin types and concentrations on the proliferation and rooting of young shoots were explored. Results: The shoots of 42-day-old seedlings were used as explants in the spring, and 1-3 shoots were formed in the explants after 7-20 days in the medium containing a certain proportion of ingredients. Auxin auxin NAA0.2-0.4mg / 1 in medium with low inorganic salts, 20-30 days to root. The research indicated that bud differentiation is closely related to cytokinin, inorganic salt content, sucrose concentration and auxin type and concentration.