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利用15对SRAP标记引物分析落花生属17份材料间的遗传变异情况。结果表明17份材料共检测出119个位点,其中多态性位点为100个,多态性百分率为89.00%,平均每对引物检测出6.67个多态性位点,扩增范围为5~10。材料间的遗传相似性范围为0.310~0.967,平均为0.666。基于遗传相似,利用UPGMA聚类分析表明,17份落花生可聚为3个类群,主成分分析析结果与聚类分析结果一致。研究结果证实了落花生种质资源遗传多样性丰富,为落花生育种工作奠定了基础。
Fifteen pairs of SRAP-labeled primers were used to analyze the genetic variation among 17 materials in the genus Arachis. The results showed that a total of 119 loci were detected in 17 cultivars, of which 100 were polymorphic and the percentage of polymorphism was 89.00%. An average of 6.67 polymorphic loci per pair of primers were detected with an amplification range of 5 ~ 10. The genetic similarity between materials ranged from 0.310 to 0.967 with an average of 0.666. Based on the genetic similarity, UPGMA cluster analysis showed that 17 peanuts could be clustered into three groups. The principal component analysis results were consistent with the cluster analysis results. The results of the study confirmed that the genetic diversity of groundnut germplasm resources is rich, which laid the foundation for the work of groundnut breeding.