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为了研究显脉羊蹄甲中抗糖尿病活性成分,采用硅胶、ODS、MCI以及Sephadex LH-20等柱色谱方法从显脉羊蹄甲95%乙醇提取物中分离得到11个酚酸类成分,其结构经多种波谱技术鉴定为6’-O-没食子酰基葡萄糖异丙苷(1)、6’-O-没食子酰基葡萄糖乙苷(2)、3,4,5-三甲氧基苯酚-1-O-β-D-(6’-O-没食子酰基)吡喃葡萄糖苷(3)、3,4,5-三甲氧基苯酚葡萄糖苷(4)、没食子酸(5)、没食子酸甲酯(6)、没食子酸乙酯(7)、原儿茶酸(8)、3,5-二甲氧基-4-羟基苯甲酸(9)、erigeside C(10)和丁香酸葡萄糖苷(11)。化合物1为新化合物,化合物2、10和11为首次从羊蹄甲属植物中分离得到,其余化合物为首次从该植物中分离得到。化合物6和8对蛋白酪氨酸磷酸酶1B(protein tyrosine phosphatase1B,PTP1B)显示较好的抑制活性,其IC50值分别为72.3和54.1μmol.L-1。
In order to study the anti-diabetic active ingredient of Artemisia annulata, eleven phenolic acids were isolated from 95% ethanol extract of Artiodactyla by using silica gel, ODS, MCI and Sephadex LH-20 column chromatography. Various spectroscopic techniques were identified as 6’-O-galloxacin isoside (1), 6’-O-gallooglucoside (2), 3,4,5-trimethoxyphenol- β-D- (6’-O-galloyl) glucopyranoside (3), 3,4,5-trimethoxyphenol glucoside (4), gallic acid (5), methyl gallate (6) , Ethyl gallate (7), protocatechuic acid (8), 3,5-dimethoxy-4-hydroxybenzoic acid (9), erigeside C (10) and syringic acid glucoside (11). Compound 1 was a new compound. Compounds 2, 10 and 11 were isolated from A. awn for the first time. The rest of the compounds were isolated from this plant for the first time. Compounds 6 and 8 showed good inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) with IC50 values of 72.3 and 54.1 μmol·L-1, respectively.