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The method of gene transfer into corneal endothelium was investigated to provide a foundation for the study of TGF-β1 gene transfer to inhibit corneal graft rejection.Two days after direct injection of p MAM TGF-β1 mediated by liposom e into the anterior cham ber of rabbits,one half of corneas were made into paraffin slides and the endothelial layer was carefully torn from the other half to make a single layer slide of endothelia.By means of im munohistochemical technique, the plasmid p MAM TGF- β1 expression product TGF- β1 in the endothelia was detected.Specific TGF- β1 expression was positive in the endothelia on both the paraffin slide and the single layer slide.The results showed that by direct injection into the anterior cham ber,foreign plasmid DNA could be transferred into the endothelia and its expression was obtained.This may provide a foun- dation for further study on TGF-β1 participating in local induction of corneal imm une tolerance.
The method of gene transfer into corneal endothelium was investigated to provide a foundation for the study of TGF-β1 gene transfer to inhibit corneal graft rejection. Two days after direct injection of p MAM TGF-β1 mediated by liposom e into the anterior cham ber of rabbits, one half of corneas were made into paraffin slides and the endothelial layer was carefully torn from the other half to make a single layer slide of endothelia. By means of im munohistochemical technique, the plasmid p MAM TGF- β1 expression product TGF- β1 in the endothelia was detected. The specific TGF-β1 expression was positive in the endothelia on both the paraffin slide and the single layer slide. The results showed that by direct injection into the anterior cham ber, foreign plasmid DNA could be transferred into the endothelia and its expression was obtained. This may provide a foun- dation for further study on TGF-β1 participating in local induction of corneal imm une tolerance.