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取１ｍｍ柑桔嫁接树茎尖在附加６－ＢＡ（１．０ｍｇ／Ｌ）和ＮＡＡ（０．４ｍｇ／Ｌ）的ＭＳ培养基上具有良好的分化效果；不同梢期的茎尖，其分化率不同。休眠结束后，刚开始萌发的芽或盛长期的芽最适合作茎尖培养的起始外植体。在以上分化培养基上，其茎尖萌发率可达７８．４％～８５．７％，且生长健壮；不同品种的柑桔茎尖在同一增殖培养基上，其增殖系数有较大差异；经连续继代增殖的芽转入改良的ＭＳ培养基中，进行壮苗培养，可获得健壮植株；采用１００ｍｇ·ｋｇ－１ＩＢＡ浸泡新梢基部，有利于生根。培养过程中，选择最适激素浓度，蔗糖浓度，培养基酸碱度以及足够的光照，可有效地避免试管苗黄化和玻璃状苗的出现。
The stem tips of 1 mm citrus grafted tree had good differentiation effect on MS medium supplemented with 6-BA (1.0 mg / L) and NAA (0.4 mg / L). The shoot apical differentiation rate different. At the end of dormancy, buds or long-term buds that have just started to germinate are best suited as starter explants for shoot tip culture. In the above differentiation medium, the shoot tip germination rate of 78.4% ~ 85.7%, and grow robust; different varieties of citrus shoot tips in the same proliferation medium, the proliferation coefficient is quite different; Successive generations of the proliferation of buds transferred to the modified MS medium, strong seedling culture, to obtain robust plants; soaking with 100mg · kg-1IBA shoot tip is conducive to rooting. In the process of culture, the optimum hormone concentration, sucrose concentration, medium pH and enough light were chosen to avoid the yellowing of plantlets and the appearance of vitrify seedlings.