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目的:为探讨胞内、外钙离子是否在水杨酸诱导丹参迷迭香酸生物合成中起作用。方法:以生长50 d的丹参幼苗为材料,通过喷施水杨酸诱导迷迭香酸合成量增加,再利用胞外钙通道抑制剂verapamil(Vp)和LaCl3、胞内钙调素拮抗剂trifluoperazine(TFP)和胞内钙通道上IP3受体抑制剂LiCl处理,分别考察水杨酸、钙离子通道抑制剂/钙调素拮抗剂处理后迷迭香酸的合成量及其合成相关酶(PAL,TAT)的活性变化。结果:2.0 mmol·L-1的水杨酸处理24 h后可诱导迷迭香酸合成量升高到(40.51±2.16)mg·g-1,是对照的1.97倍,迷迭香酸合成相关酶PAL活性升高到对照的1.42倍,TAT活性升高到对照的1.29倍;Vp,LaCl3,TFP,LiCl处理均抑制了水杨酸诱导的PAL,TAT活性的升高,从而导致了迷迭香酸合成量降低。结论:水杨酸诱导丹参迷迭香酸生物合成过程中,胞内、外钙离子发挥着重要的信号转导作用。
AIM: To investigate whether intracellular and extracellular calcium play a role in salicylic acid-induced rosmarinic acid biosynthesis in Salvia miltiorrhiza. Methods: Salvia miltiorrhiza was used to induce rosmarinic acid synthesis after 50 days of growth. The extracellular calcium channel inhibitor verapamil (Vp) and LaCl3, trifluoperazine (TFP) and LiCl, an inhibitor of IP3 receptor on the intracellular calcium channel, respectively. The synthesis of rosmarinic acid and the related enzymes of PAL synthesis after treatment with salicylic acid, calcium channel blockers and calmodulin antagonists , TAT) activity changes. Results: The amount of rosmarinic acid synthesis induced by salicylic acid (2.0 mmol·L-1) for 24 h increased to (40.51 ± 2.16) mg · g-1, which was 1.97 times that of the control The activity of PAL increased 1.42-fold and the activity of TAT increased to 1.29-fold of that of control. Vp, LaCl3, TFP and LiCl all inhibited the salicylic acid-induced increase of PAL and TAT activity, The amount of acid synthesis decreased. CONCLUSIONS: Salicylic acid induces intracellular and extracellular calcium ions to play important signal transduction roles in the biosynthesis of rosmarinic acid.