【摘 要】
：
Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromato
【机 构】
：
College of Life Sciences,The Ocean College of Hainan University,Key Laboratory of Marine Bio-active
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Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry(LC/MS), and its content accounted for 87.62% of total carotenoids (1,786 μg/g). High Performance Liquid Chromatogrephy(HPLC)analysis showed that the purity of the astaxanthin reached about 96.16% through a simple purification. Maximum astaxanthin production(1,908/μg/g)was obtained when the yeast was grown at 10℃ in seawater medium containing 5g/L sodium acetate, 5g/L peptone, 0.5g/L NaCl, 0.01g/L KH2P04;0.01g/L MgS04·7H2O and 0.001 g/L FeS04·7H20 at pH 7.5.
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