从一例52岁食管鳞癌患者的食管组织得到的原代成纤维细胞.经用化学致癌物(N-methyl-N’-nitro-N-nitrosoguanidine,MNNG)处理后。诱发了恶性转化.细胞显示恶性细胞的生物学及形态学特性,如失去接触抑制、体外无限制的生长、非整倍体性、ConA凝集性、细胞表面微绒毛形成,在琼脂固体培养基上生长,在免疫抑制的新生小鼠上接种形成肿瘤(低分化纤维肉瘤). Primary fibroblasts obtained from the esophageal tissue of a 52-year-old patient with esophageal squamous cell carcinoma were treated with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG). Induced malignant transformation. The cells showed the biological and morphological features of malignant cells, such as loss of contact inhibition, in vitro unrestricted growth, aneuploidy, ConA agglutination, cell surface microvilli formation on agar solid media Were grown and inoculated on immunosuppressed newborn mice to form tumors (poorly differentiated fibrosarcoma).