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以农作物秸秆为主要原料 ,采用微生物固体发酵方式对侧孢霉 (Sporotrichumsp) .产生木质纤维素酶进行了探讨。研究发现侧孢霉可降解作物秸秆的木质纤维 ,降解作用强 ,同时提高了发酵底物的蛋白含量。玉米秸秆、稻草、麦草及甜菜渣是发酵木质纤维素酶和单细胞蛋白的最适合发酵底物。在以玉米秸秆为原料的发酵过程中 ,发酵到第 4天菌体生长量达到最高值 ,比发酵初期的菌体生长量增加了 8倍 ;发酵第 6天培养物的粗蛋白含量由起初的 1 0 %提高到 1 7 5% ,对底物纤维降解率达到1 3 6%。侧孢霉在发酵过程中产生大量的木质纤维素酶 ,其中羧甲基纤维素酶 (CM Case)、β 葡聚糖苷酶 ( β Gase)、滤纸酶 (FPase)、木质素过氧化物酶 (LiP)和赖锰木质素过氧化物酶 (MnP)在本实验中进行了检测。结果表明 ,最大酶活性的产生时间是在发酵的第 4到第 6天之间 ,其酶活性分别为 :羧甲基纤维素酶 2 40 3 1U/ g ,β 葡聚糖苷酶 86 7U/ g ,滤纸酶 1 3 4 8U/ g ,木质素过氧化物酶 1 4 4U/ g ,赖锰木质素过氧化物酶 1 7 67U/ g。
The crop straw was used as the main raw material, and the microorganism solid fermentation method was used to study the production of lignocellulase against Sporotrichum sp. The study found that Aspergillus oryzae could degrade the wood fiber of crop stalks and had a strong degradation effect and at the same time increased the protein content of the fermentation substrate. Corn stover, straw, wheat straw and beet pulp are the most suitable fermentation substrates for the fermentation of lignocelluloses and single cell proteins. In the fermentation process using corn stalk as raw material, the amount of growth reached the highest value on the 4th day after fermentation, which increased by 8 times than that of the initial stage of fermentation. The crude protein content on the 6th day of fermentation was increased from the initial 10% to 17.5%, the substrate fiber degradation rate of 136%. Aspergillus oryzae produces a large amount of lignocellulose during the fermentation process, among which, CM Case, β Gase, FPase and lignin peroxidase LiP) and manganese-dependent lignin peroxidase (MnP) were tested in this experiment. The results showed that the maximum enzyme activity was produced between the 4th and 6th days of fermentation. The enzyme activity was 2 40 3 1U / g, β-glucanase 86 7U / g , Filter paper enzyme 1 34 U / g, lignin peroxidase 1 4 4U / g, Lai manganese lignin peroxidase 1 7 67U / g.