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通过检测胃肠道癌症患者肿瘤中心组织、癌旁组织的端粒酶活性，探讨肿瘤手术切缘的安全性。方法分别取肿瘤中心组织及肿瘤上下切缘各２ｃｍ、４ｃｍ组织共５处，用ＴＲＡＰ银染显色法检测各部分端粒酶的活性。结果１５例胃癌中端粒酶阳性９３．３％。癌旁距肿瘤边缘２ｃｍ，阳性２０％，４ｃｍ以上均为阴性。１０例直肠癌中端粒酶阳性率为８０％，癌旁距肿瘤２ｃｍ阳性率为１０％，４ｃｍ以上皆为阴性。结论肿瘤的中心部分与边缘组织的端粒酶活性存在显著性差异，而且与离肿瘤的距离有关，
Through the detection of telomerase activity in tumor center tissues and adjacent tissues of gastrointestinal cancer patients, the safety of surgical margins of tumors was explored. Methods A total of 5 specimens of 2 cm and 4 cm sections from the tumor center tissue and the upper and lower tumor margins were obtained. The activity of telomerase in each section was detected by TRAP silver staining. Results The positive rate of telomerase was 93.3% in 15 cases of gastric cancer. The tumor was 2 cm from the edge of the tumor, 20% positive, and 4 cm or more negative. The positive rate of telomerase in 10 cases of rectal cancer was 80%, and the positive rate of tumor adjacent to tumor 2cm was 10%, and 4 cm or more were all negative. Conclusion There is a significant difference in the telomerase activity between the central part of the tumor and the marginal tissue, and it is related to the distance from the tumor.