论文部分内容阅读
利用开顶式气室(OTCs)对大豆进行臭氧(O3)胁迫试验,研究了高浓度O3对大豆根系体积、根瘤数、根瘤干重、根干重等根系形态指标以及膜脂过氧化程度、活性氧、抗氧化酶活性等活性氧代谢指标的影响。结果表明:在高浓度O3处理下,根系体积、根及根瘤干重、根瘤数降低,根系MDA含量、相对电导率显著上升,膜脂过氧化程度加剧;超氧阴离子(O2.-)产生速率和H2O2含量增加;O3胁迫处理的SOD活性在分枝期高于CK处理,其余各时期均低于CK处理;CAT活性除在开花期低于CK处理外,其它时期均高于CK处理;POD活性呈先升后降的趋势,各个时期均高于CK处理。高浓度O3导致大豆抗氧化系统的氧化损伤,对大豆表现为伤害效应,从而抑制了大豆根系的正常生长发育。
Ozone (O3) stress experiments were conducted on soybean using OTCs. The effects of high concentration of O3 on root morphology, root nodule number, root nodule dry weight, root dry weight and other root morphological indexes and membrane lipid peroxidation were studied. Reactive oxygen species, antioxidant enzyme activity and other indicators of reactive oxygen species. The results showed that under high concentration of O3, root volume, root and nodule dry weight, number of nodules decreased, root MDA content and relative conductivity increased significantly, and the degree of membrane lipid peroxidation increased. The production rate of O 2 - And the content of H2O2 increased. The SOD activity under O3 stress was higher than that under CK treatment at branching stage, but lower than CK under the other stages. CAT activity was higher than CK under flowering stage except CK, POD Activity was first increased and then decreased, all periods were higher than the CK treatment. High concentrations of O3 lead to oxidative damage of soybean antioxidant system, showing damaging effects on soybean, thus inhibiting the normal growth and development of soybean roots.