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目的:建立以反相高效液相色谱法测定人血浆中氟比洛芬浓度的方法。方法:采用外标法,以乙腈沉淀法处理样品后进样测定,色谱柱为Symmetry shield C_(18),流动相为磷酸盐缓冲液(pH7.0):乙腈=75:25,流速为1.0 mL·min~(-1),紫外检测波长为247 nm。结果:氟比洛芬血药浓度在0.05～20μg·mL~(-1)范围内线性关系良好(r=0.999 1),检测限为0.05μg·mL~(-1);低、中、高浓度的回收率在96.1%～107.1%之间,日内及日间RSD均<10%,符合方法学要求。结论:本方法简便、准确、快速,适用于人血浆中氟比洛芬浓度监测及药动学研究。
Objective: To establish a method for the determination of flurbiprofen in human plasma by reversed-phase high performance liquid chromatography. Methods: The sample was treated with acetonitrile by precipitation method. The column was Symmetry shield C_ (18). The mobile phase consisted of phosphate buffer (pH7.0): acetonitrile = 75:25, the flow rate was 1.0 mL · min ~ (-1), UV detection wavelength of 247 nm. Results: The linear range of flurbiprofen was 0.05 ~ 20μg · mL -1 (r = 0.999 1), the limit of detection was 0.05 μg · mL -1, while the low, middle and high The recoveries were between 96.1% and 107.1% with RSDs of <10% during day and day, which met the requirements of methodology. Conclusion: The method is simple, accurate and rapid and suitable for the monitoring of flurbiprofen concentration and pharmacokinetics in human plasma.