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为探讨细胞骨架在回肠上皮和抗原呈递细胞的表达,用细胞骨架的单克隆抗体对日本正常雌性兔的回肠石蜡切片进行免疫组织化学ABC法染色。结果表明,波形蛋白(vimentin)表达于回肠淋巴滤泡上皮中的微皱褶细胞(M细胞)和血管内皮;微管蛋白(α-tubulin)表达于M细胞、柱状吸收细胞和血管内皮;肌动蛋白(actin)表达于M细胞、柱状吸收细胞和血管内皮和平滑肌、结缔组织的一些细胞;细胞角蛋白-18(cytokeratin-18)表达于肠绒毛上皮的纹状缘。本文对细胞骨架的意义进行了讨论。
To investigate the expression of cytoskeleton in ileal epithelium and antigen-presenting cells, immunohistochemical ABC method was used to stain the ileal paraffin sections of normal female rabbits with cytoskeleton monoclonal antibody. The results showed that vimentin was expressed in micro-follicular cells (M-cells) and vascular endothelium in ileal lymphoid follicular epithelium; α-tubulin was expressed in M cells, columnar absorptive cells and vascular endothelium; muscle Actin is expressed in M cells, columnar cells and vascular endothelial and smooth muscle, connective tissue cells; cytokeratin-18 (cytokeratin-18) expression in the villi of the villi of the villi. This article discusses the implications of cytoskeleton.