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背景研究发现骨髓基质细胞移植后能够游走到全身不同部位,增生转化为相应细胞,能不同程度恢复损伤器官功能。以证实骨髓基质细胞可在体外转化成中胚层和内胚层细胞。目的探讨骨髓基质细胞体外转化为神经细胞的可能性。设计设立对照的重复测量设计。地点和对象哈尔滨医科大学附属第一医院骨科。健康雄性SD大鼠,120～150g,清洁级,由哈尔滨医科大学附属第一医院动物室提供。干预骨髓基质细胞原代培养、传代后使用3种不同方法诱导。行大体观察,免疫组织化学及细胞电生理检查和长期培养研究。主要结局观察指标骨髓基质细胞诱导、细胞免疫组化和细胞电生理检测结果。结果第1诱导方案加入诱导剂1h50%转化成神经细胞,90min70%～80%转化为神经细胞。第2诱导方案加入诱导剂24h10%转化成神经细胞。第3诱导方案BMSC40%加入,24h90%转化成神经细胞,胞体小突触短。BMSC80%时加入,转化的神经细胞呈巢状排列占40%。骨髓基质细胞体外可诱导为突触明确的神经细胞(诱导率>80%);诱导后细胞表面有神经特异性抗原NSE,GFAP表达;同时具有早期神经细胞电流特性。结论骨髓基质细胞可横向转化为早期神经细胞。
Background study found that bone marrow stromal cells can migrate to different parts of the body after transplantation, hyperplasia into the corresponding cells, to varying degrees, restore organ function. To confirm that bone marrow stromal cells can be transformed into mesoderm and endoderm cells in vitro. Objective To investigate the possibility of bone marrow stromal cells transformed into nerve cells in vitro. Design a set of duplicate measurement designs. Location and object Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University. Healthy male SD rats, 120 ~ 150g, clean level, provided by the First Affiliated Hospital of Harbin Medical University animal room. Intervention of bone marrow stromal cells primary culture, after passage using three different methods of induction. Line general observation, immunohistochemistry and cell electrophysiology and long-term culture studies. The main outcome indicators of bone marrow stromal cell induction, cell immunohistochemistry and cell electrophysiological test results. Results The first induction program by adding inducer 1h50% into nerve cells, 90min 70% ~ 80% into nerve cells. The second induction program by adding inducer 24h10% into nerve cells. Third induction program BMSC40% added, 24h90% into nerve cells, cell synapses short. When BMSC was added at 80%, the transformed nerve cells were nested for 40%. Bone marrow stromal cells can be induced into synapse clear neural cells (induction rate> 80%) in vitro; the neural cell surface NSE and GFAP expression induced; at the same time with early neural cell current characteristics. Conclusion Bone marrow stromal cells can be transversally transformed into early neural cells.