采用SD未成年大鼠在标准条件下饲养,至26天早晨注射10IU孕马血清促性腺激素。两天后引起卵泡发育及排卵。在28天内原性LH高峰到来之前3～6h断头处死,放血并切除卵巢。在立体显微镜下,自卵巢分离8～10个最大的卵泡。每次实验需用100个卵泡,每5个卵泡置入一个长颈孵育瓶内(瓶内备有1ml37℃ Gibco氏MEM培养液),孵 SD minors were housed under standard conditions and 10IU pregnant horse serum gonadotropin was injected into the morning of 26 days. Two days after causing follicular development and ovulation. In 28 days before the onset of primary LH peak 3 ~ 6h decapitated, bleeding and ovariectomy. Under a stereomicroscope, 8 to 10 of the largest follicles were isolated from the ovary. Each experiment requires 100 follicles, every 5 follicles into a long-necked flask (1ml 37 ℃ Gibco’s MEM medium) and incubate