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目的:观察损伤血管后碱性成纤维细胞生长因子(bFGF)的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉后再狭窄的干预机制。方法:随机将160只雄性SD大鼠分为假手术组,阴性对照组,益气活血方大、中、小剂量组。首先自左颈外动脉球囊损伤大鼠左颈总动脉,后给予高脂饲料喂养30天,造成动脉硬化,然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉再狭窄。手术前1天开始给药,每组动物于术后(3天、7天、14天、28天)处死,取损伤颈总动脉,常规固定、包埋、切片,分别做HE染色和免疫组织化学SABC法检测bFGF的表达。结果:血管损伤后第14天、28天用药组bFGF表达量与阴性对照组比较有显著性差异(P<0.05)。结论:益气活血方对球囊损伤后大鼠颈动脉再狭窄有明显的防治作用,其机制可能与下调bFGF的表达,抑制血管平滑肌细胞增殖有关。
OBJECTIVE: To observe the expression of basic fibroblast growth factor (bFGF) after vascular injury, and to explore the mechanism of Yiqi Huoxue decoction on carotid artery stenosis after balloon injury in rats. Methods: 160 male SD rats were randomly divided into sham operation group, negative control group, Yiqi Huoxue Fang large, medium and small dose group. First of all, the left carotid artery was injured from the left external carotid artery and then fed with high-fat diet for 30 days to cause atherosclerosis. Then, the right common carotid artery punctured the balloon and dilated the left common carotid artery to cause the common carotid artery Restenosis. One day before the surgery, the animals were sacrificed after the operation (3 days, 7 days, 14 days and 28 days). The common carotid arteries were fixed, embedded, and sectioned for HE staining and immunohistochemistry Chemical SABC method was used to detect the expression of bFGF. Results: Compared with the negative control group, the expression of bFGF in the 14th day and 28th day after the vascular injury was significantly different (P <0.05). Conclusion: Yiqi Huoxue Prescription has obvious preventive and therapeutic effects on carotid restenosis after balloon injury in rats. The mechanism may be related to down-regulating the expression of bFGF and inhibiting the proliferation of vascular smooth muscle cells.