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目的优选大孔吸附树脂分离纯化蜘蛛香中环烯醚萜类成分。方法采用高效液相色谱法测定蜘蛛香中环烯醚萜类成分11-ethoxyviburtinal的含量,并以此为指标,采用单因素考察、正交试验设计法对上样溶液浓度、洗脱溶剂、洗脱流速等进行考察及优化。结果 AB-8大孔吸附树脂具有良好的吸附分离性能。确定工艺参数为:上样浓度为0.2 g/mL,树脂柱径高比为1∶6,除杂溶剂为2倍柱体积(BV)的30%乙醇,洗脱溶剂为90%乙醇,洗脱体积为5 BV,洗脱流速为4 BV/h。结论此法操作方便,为大生产提取分离纯化蜘蛛香提供了依据。
Objective To optimize the separation and purification of iridoids from spider incense by macroporous adsorption resin. Methods The content of 11-ethoxyviburtinal of iridoid in Spider incense was determined by high performance liquid chromatography (HPLC). Using single factor test and orthogonal experimental design, the concentration of the sample solution, the elution solvent, Flow rate and other inspection and optimization. Results AB-8 macroporous resin has good adsorption and separation performance. The process parameters were determined as follows: the sample concentration was 0.2 g / mL, the resin column diameter to height ratio was 1: 6, the impurity removal solvent was 2 column volumes (BV) of 30% ethanol, the elution solvent was 90% ethanol, The volume is 5 BV and the elution flow rate is 4 BV / h. Conclusion This method is easy to operate and provides the basis for large-scale extraction and purification of spider incense.