论文部分内容阅读
目的 观察表皮生长因子 (EGF)对接受长期全肠外营养 (TPN)的腹部辐射大鼠肠道细菌移位的影响 ,探讨其机制 .方法 应用 TSB培养、图像分析、高效液相色谱等方法检测应用 EGF后大鼠死亡率 ,肠粘膜形态结构 ,肠道细菌移位 ,血浆及组织谷氨酰胺 (Gln)水平及肠道 Gln摄取率 .结果 应用 EGF后 ,大鼠死亡率显著降低 ,肠粘膜结构屏障改善 ,细菌移位减少 ,血液及组织 Gln水平提高 .结论 EGF在长期TPN显著改善辐射性肠粘膜屏障损伤大鼠营养的同时 ,减少肠道细菌移位 ,显著改善血液及组织的 Gln代谢 ,维持肠粘膜上皮的正常功能 ,加速修复损伤的肠粘膜屏障 ,降低死亡率 .
Objective To investigate the effect of epidermal growth factor (EGF) on intestinal bacterial translocation in rats with long-term total parenteral nutrition (TPN) irradiation and to explore the possible mechanism.Methods TSB culture, image analysis and high performance liquid chromatography After the application of EGF, the mortality rate, intestinal mucosa morphology, intestinal bacterial translocation, plasma and tissue glutamine (Gln) level and intestinal Gln uptake rate were all significantly decreased.Results After EGF treatment, the mortality of rats was significantly decreased, the intestinal mucosa The structural barrier was improved, the bacterial translocation was decreased, and the blood and tissue Gln level was increased.Conclusion EGF can significantly reduce the intestinal bacterial translocation and significantly improve the Gln metabolism in blood and tissues while long-term TPN significantly improves the nutrition of rats with radiation-induced intestinal mucosal barrier injury , To maintain the normal function of intestinal epithelial, accelerate the repair of damaged intestinal mucosal barrier, reduce mortality.