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目的通过观察肺鳞状细胞癌及癌旁肺组织中人乳头瘤病毒（humanpapillomavirus，HPV）的感染、分布、存在状态及与临床病理资料间的关系，探讨HPV感染在肺鳞状细胞癌发病中的作用。方法用共有引物PCR检测120例肺鳞状细胞癌及癌旁肺组织中HPV的感染率，用多重PCR对阳性标本的HPV6/11．16．18分型，用原位杂交方法观察HPV在肺鳞癌组织中的分布及存在状态。结果肺鳞癌组织HPV感染率（35％）显著高于癌旁肺组织（67％）（P＜0．01），HPV在肺鳞癌组织中可能以游离和整合两种状态存在并可在肺鳞癌组织中表达，早期（Ⅰ期）肺鳞癌HPV感染率（44.3％）显著高于进展期（Ⅱ～Ⅲ期）肺鳞癌（25．4％）（P＜0．05），重度吸烟组HPV感染率（44．7％）显著高于非吸烟组（18．2％）（P＜0．01）。结论HPV感染在肺鳞癌的发病中可能起一定作用，肺鳞癌组织中HPV感染与吸烟有关。
Objective To investigate the relationship between human papillomavirus (HPV) infection, distribution, and presence status and clinicopathological data in lung squamous cell carcinoma and adjacent lung tissues to investigate the role of HPV infection in the pathogenesis of lung squamous cell carcinoma. The role. Methods HPV infection rates in 120 cases of lung squamous cell carcinoma and adjacent lung tissues were detected by PCR with consensus primers. HPV6/11.16.18 typing of positive specimens was performed by multiplex PCR. HPV was observed in lungs by in situ hybridization. The distribution and presence of squamous cell carcinoma. Results HPV infection rate in lung squamous cell carcinoma tissue (35%) was significantly higher than that in para-tumor lung tissue (67%) (P<0.01). HPV may be present in both squamous cell carcinoma tissues in both free and integrated states and may be present in In lung squamous cell carcinoma tissues, HPV infection rate (44.3%) in early (stage I) lung squamous cell carcinoma was significantly higher than that in advanced stage (II-III) lung squamous cell carcinoma (25.4%) (P<0.05). The infection rate of HPV in severe smoking group (44.7%) was significantly higher than that in non-smoking group (18.2%) (P<0.01). Conclusion HPV infection may play a role in the pathogenesis of lung squamous cell carcinoma. HPV infection in lung squamous cell carcinoma is related to smoking.