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目的：探讨维拉帕米在光动力学治疗结肠癌中增效作用及机制。方法：在结肠癌细胞培养的基础上，采用荧光分光光度法、ＭＴＴ法、荧光探针法等测定维拉帕米增效后癌细胞内游离钙、癌细胞存活率和细胞内光敏剂含量的变化。结果：维拉帕米可显著增加癌细胞内光敏剂含量和光动力学疗法（ＰＤＴ）杀伤作用，激光照射后癌细胞存活率与细胞内光敏剂含量呈显著负相关。癌细胞内光敏剂在８～１６ｈ即可达到较高浓度。加维拉帕米后，癌细胞内游离钙降低，激光照射后游离钙明显增加，与癌细胞损伤程度一致。结论：维拉帕米在ＰＤＴ治疗大肠癌中具有明显的增效作用，胞内游离钙起重要作用。
Objective: To investigate the synergistic effect and mechanism of verapamil in photodynamic therapy of colon cancer. METHODS: On the basis of colon cancer cell culture, fluorescence spectrophotometry, MTT assay, and fluorescent probe method were used to determine the intracellular free calcium, cancer cell survival rate and intracellular photosensitizer content after verapamil boosting. Variety. RESULTS: Verapamil significantly increased the photosensitizer content in cancer cells and photodynamic therapy (PDT) killing. The survival rate of cancer cells after laser irradiation was significantly negatively correlated with the content of photosensitizers in cells. Photosensitizers in cancer cells can reach higher concentrations within 8 to 16 hours. After Gavilapamil, the intracellular free calcium decreased, and the free calcium increased significantly after laser irradiation, consistent with the degree of cancer cell damage. CONCLUSION: Verapamil has a significant synergistic effect in the treatment of colorectal cancer with PDT, and intracellular free calcium plays an important role.