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样品溶液的制备及分析方法称取粉碎后样品500mg加50％甲醇40ml在水浴上回流提取1h,趁热过滤,滤液和洗液合并至50ml,取20ml通过填充了CM-(?)CH的柱(12mm×30mm),该柱用50％甲醇20ml清洗后,用0.1mol/LHCL洗脱并定容至20ml为样品溶液。内标溶液为L-肾上腺素的0.1mol/L HCI溶液(0.05mg/ml)。分析时取样品和标准溶液各1.0ml,加入内标溶液1.0ml,进样20μl。分析条件为:柱3011-C(弱酸性阳离子交换树脂4×250mm),流动相:0.1mol/L KH_2PO_4-0.3mol/L NH_4Cl-MeOH(35:35:30),流量1.0ml/min,检测220nm。在上述条件下,肾上
Preparation of sample solution and analysis method Weigh comminuted sample 500mg plus 50% methanol 40ml reflux on a water bath extract 1h, while hot filtration, filtrate and washings were combined to 50ml, take 20ml through the column filled with CM-(?) CH (12 mm×30 mm), the column was washed with 20 ml of 50% methanol, and eluted with 0.1 mol/L HCL and set to 20 ml as a sample solution. The internal standard solution was L-adrenergic 0.1 mol/L HCI solution (0.05 mg/ml). For analysis, take 1.0 ml of each sample and standard solution, add 1.0 ml of internal standard solution, and inject 20 μl. Analytical conditions were: column 3011-C (weakly acidic cation exchange resin 4 x 250 mm), mobile phase: 0.1 mol/L KH 2 PO 4 -0.3 mol/L NH 4 Cl-MeOH (35:35:30), flow rate 1.0 ml/min, detection 220nm. Under the above conditions, on the kidney