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MicroRNAs(miRNAs) are small,non-coding RNAs that negatively adjust gene expression in multifarious biological processes.However,the regulatory effects of miRNAs on Schwann cells remain poorly understood.Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction,thereby affecting proliferation and migration of Schwann cells.This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1(Cand1).Up-regulated expression of miR-148b-3p promoted Schwann cell migration,whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro.Further experiments confirmed that Candl was a direct target of miR-148b-3p,and Candl knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration.These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Candl in vitro.
MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction , thereby affecting proliferation and migration of Schwann cells. This study conducted whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1) .Up-regulated expression of miR-148b-3p promoted Schwann cell migration, but silencing of miR-148b-3 inhibited Schwann cell migration in vitro. Flow PCR confirmed that Candlc was a direct target of miR-148b-3p, and Candl knockdown was suppressed on miR-148b-3p inhibitor on Schwann cell migration. These results suggest that miR-148b-3p promoted migration of Schwann cells by directly targeting Candl in vitro.