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目的探讨甲泼尼龙对放射性肺损伤的防治作用。方法将雄性新西兰兔45只,随机分为空白对照组(A)、单纯照射组(B)和照射加甲强龙组(C),每组15只。A组静脉注射生理盐水0.1mL·kg-1·d-1,连续4周;B组右肺单纯照射;C组照射开始时腹腔注射甲泼尼龙,0.4mg·kg-1·d-1,连续4周。采用酶联免疫吸附试验(ELISA)测量照射前及照射后第1、3、5个月动物血清TGF-β1的含量。结果A组TGF-β1含量1~5个月差异无统计学意义;B组第3个月时最高,5个月时较3个月时下降;C组自第3个月时亦开始升高。3个月时,TGF-β1含量B组明显高于C组(P<0.05);C组明显高于A组(P<0.05)。结论甲泼尼龙可以通过抑制TGF-β1的表达,从而减轻了放射性肺损伤。
Objective To investigate the preventive and therapeutic effects of methylprednisolone on radiation-induced lung injury. Methods 45 male New Zealand white rabbits were randomly divided into blank control group (A), irradiation group (B) and irradiation plus methylprednisolone group (C), 15 rats in each group. Rats in group A were injected with 0.1 mL · kg-1 · d-1 of normal saline for 4 weeks, group B received right lung irradiation, group C received intraperitoneal injection of methylprednisolone (0.4 mg · kg-1 · d-1) For 4 weeks. Serum TGF-β1 levels were measured before and after irradiation with enzyme-linked immunosorbent assay (ELISA). Results There was no significant difference in the content of TGF-β1 between 1 and 5 months in group A, the highest at the third month in group B, the third month at 5 and the third month in group C . At 3 months, the content of TGF-β1 in group B was significantly higher than that in group C (P <0.05), and in group C was significantly higher than that in group A (P <0.05). Conclusion Methylprednisolone can attenuate radiation-induced lung injury by inhibiting the expression of TGF-β1.