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目的研究体外培养的大鼠纹状体神经干细胞在植入同种视网膜后,向少突胶质细胞分化并产生髓鞘的过程,观察髓鞘形成对视网膜结构的可能影响,建立一种中枢神经髓鞘体内发育的新模型。方法将体外传代培养的胚胎纹状体神经干细胞植入新生大鼠的玻璃体腔内,在移植后不同时期观察视网膜内髓鞘的出现部位以及扩展趋势,利用不同染色方法对标本进行检验分析,利用透射电镜进行超微结构观察。结果移植细胞4周后部分视网膜内开始出现成束髓鞘,只分布于神经纤维层。髓鞘束出现的比例、分布面积和形态变化与移植后动物的存活时间相关。电镜观察可见节细胞轴突外包绕有结构正常的中枢神经样髓鞘。较厚的髓鞘束对视网膜节细胞的分布产生一定影响。结论大鼠纹状体神经干细胞可在同种视网膜内向成熟少突胶质细胞分化,视网膜神经纤维层具有促使髓鞘形成的作用;本实验可能为研究少突胶质细胞分化及髓鞘生成机制提供新的在体模型。
OBJECTIVE: To study the process of differentiation of neural stem cells into oligodendrocytes and the generation of myelin after implantation of the same kind of retina in rat striatum. To observe the possible influence of myelination on the structure of retina and to establish a central nervous system New model of myelin development in vivo. Methods Embryonic striatal neural stem cells cultured in vitro were implanted into the vitreous cavity of newborn rats. The appearance of the myelin sheath in the retina was observed at different stages after transplantation. The specimens were examined by different staining methods. Transmission electron microscope for ultrastructure observation. Results After 4 weeks, some myelin sheaths began to appear in part of the retina and only distributed in the nerve fiber layer. The proportions, distribution areas and morphological changes of myelin sheaths correlated with the survival time of the animals after transplantation. Electron microscopy showed that the axons of the ganglion cells were surrounded by a normal central nervous system myelin sheath. Thicker myelin bundles have some influence on the distribution of retinal ganglion cells. Conclusion Rat NSCs can differentiate into mature oligodendrocytes in the same kind of retina, and retinal nerve fiber layer can promote the formation of myelin. This study may be useful for studying the mechanism of oligodendrocyte differentiation and myelination Provide new in-body models.