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目的探讨PCR法检测肺炎支原体(Mycoplasma pneumoniae,Mp)的临床应用价值。方法选择213例社区获得性肺炎(community-acquired pneumonia,CAP)患者中临床证实的62例肺炎支原体感染者的咽拭子、血清标本,分别经肺炎支原体核酸PCR(Polymerase chain reaction,PCR)扩增和血清学检测(IgM)、肺炎支原体培养,采用四格表χ2检验比较三种方法的检测结果。结果三种方法检测肺炎支原体(Mycoplasma Pneumoniae,MP)的阳性率分别为87.1%、72.6%、45.2%,其中PCR法与血清法比较无统计学差异(P>0.05);与培养法比较有统计学差异(P<0.01);血清法与培养法比较有统计学差异(P<0.01)。结论PCR法检测肺炎支原体具有比培养法、血清法更高的敏感性和特异性,特别是对于早期的感染,对于临床早期的诊断和治疗具有重要意义。
Objective To investigate the clinical value of PCR in the detection of Mycoplasma pneumoniae (Mp). Methods Totally 62 throat swabs and serum samples from 213 patients with community-acquired pneumonia (CAP) who were clinically confirmed by Mycoplasma pneumoniae infection were enrolled in this study. They were amplified by polymerase chain reaction (PCR) And serological tests (IgM), Mycoplasma pneumoniae culture, the use of four tables χ2 test to compare the results of three methods. Results The positive rates of Mycoplasma Pneumoniae (MP) were 87.1%, 72.6% and 45.2%, respectively. There was no significant difference between PCR and serum method (P> 0.05) (P <0.01). There was a significant difference between serum and culture methods (P <0.01). Conclusion The detection of mycoplasma pneumoniae by PCR method has higher sensitivity and specificity than the culture method and serum method, especially for the early infection, which is of great significance for early clinical diagnosis and treatment.