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目的 :建立测定复方荔枝草颗粒剂中高车前苷含量的高效液相色谱法。方法 :以KF -C18(2 5 0mm× 4 6mm ,10 μm)为分析柱 ,流动相为甲醇 -水 -pH 3 5的 1 0mol·L-1磷酸盐缓冲液 (4 0∶5 5∶5 ) ;柱温为 45℃ ;检测波长为 335nm ;流速为 1 0mL·min-1,采用面积外标法。结果 :线性范围 3 12× 10 -3 ～ 0 2 0 μg ,平均回收率为 96 96 %～ 10 1 5 % ,RSD <0 6 9% (n =3)。结论 :本法简便、灵敏、重现性好 ,可用于测定该颗粒剂高车前苷的含量和质量标准的控制。
Objective : To establish high performance liquid chromatography (HPLC) method for the determination of genistein in compound litchi granules. METHODS: KF-C18 (250 mm×4 6 mm, 10 μm) was used as the analytical column. The mobile phase consisted of methanol-water-pH 3 5 in 10 μmol·L-1 phosphate buffer (4:5 5:5). The column temperature was 45°C; the detection wavelength was 335 nm; the flow rate was 10 mL·min-1, and the area external standard method was used. Results: The linear range was 3 12×10 -3 -0.20 μg. The average recovery was 96 96% - 10 15%. The RSD was less than 0.69% (n = 3). Conclusion : This method is simple, sensitive and reproducible. It can be used to determine the content and quality standard of high antebelloid glycosides of this granule.