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本文提供一种可定量的评价光敏作用的方法。大鼠腹腔接种Walker-256肿瘤细胞后5天,按10mg/kg体重腹腔注射“激光3号”和PhotofrinⅡ,每药3鼠,24小时后处死动物,取每巴1ml腹水合并,生理盐水洗2次后将瘤细胞悬于生理盐水中,计瘤细胞数。配成1×10~4/m1细胞悬液,用日本MPF-4型荧光分光光度计测含癌细胞溶液的发射光谱,以相对荧光强度比较癌细胞对“激光3号”和PhotofrinⅡ的摄取。结果表明
This article provides a method for quantitative assessment of photosensitivity. Five days after the rats were intraperitoneally inoculated with Walker-256 tumor cells, 10 mg/kg body weight was intraperitoneally injected with “Laser 3” and Photofrin II, 3 mice per drug. After 24 hours, the animals were sacrificed, and 1 ml of ascites per bar was combined to wash with physiological saline. After that, the tumor cells were suspended in saline and the number of tumor cells was counted. A 1×10~4/m1 cell suspension was prepared and the emission spectrum of the cancer cell-containing solution was measured using a Japanese MPF-4 fluorescence spectrophotometer. The uptake of “Laser 3” and PhotofrinII by the cancer cells was compared with the relative fluorescence intensity. The results show