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采用18对SSR引物和15对AFLP引物对我国196份甘蔗常用亲本的遗传相似性进行分析,分别获得438和1192个标记,多态性位点比例分别是87.60%,80.80%,平均为84.2%;亲本遗传相似系数变幅介于0.4761～0.9099之间,总平均值为0.6673,遗传差异处于中等水平的亲本占了大多数,而差异较小和较大的都只是少数;45个甘蔗品种亲系的遗传相似系数分布在0.5687～0.6723,平均值为0.6723,91.11%甘蔗品种亲系的遗传相似系数集中在0.6100～0.7300之间,较理想的甘蔗杂交组合亲本间的遗传相似系数应为0.6723±0.0600;聚类分析表明,以遗传相似系数阈值约为0.700划分,育出45个品种所使用的30个亲本可分为9大类,在不同类型间选择亲本搭配的组合更容易选育出优良品种。
18 pairs of SSR primers and 15 pairs of AFLP primers were used to analyze the genetic similarity of 196 common sugarcane parents in our country, 438 and 1192 markers were obtained respectively, the percentage of polymorphic loci was 87.60% and 80.80% respectively, with an average of 84.2% ; The genetic similarity coefficient of the parents ranged from 0.4761 to 0.9099 with a total average of 0.6673, while the parents with the same genetic level accounted for the majority, while those with smaller and larger differences were only a minority; The genetic similarity coefficients of the lines ranged from 0.5687 to 0.6723 with the average values of 0.6723 and 91.11%, respectively. The genetic similarity coefficients of the parents of the sugarcane varieties ranged from 0.6100 to 0.7300. The genetic similarity coefficients of the desirable parents of the sugarcane hybrid combinations should be 0.6723 ± 0.0600. The cluster analysis showed that the 30 parents of 45 cultivars could be divided into 9 groups with the genetic similarity coefficient of about 0.700, and it was easier to select excellent parents by selecting parents with different combinations Variety.