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目的建立冬虫夏草和北虫草中腺苷和虫草素含量测定的方法。方法样品用水超声提取,HPLC-UV法同时测定该2种成分。色谱条件为:Eclipse-C18色谱柱(4.6 mm×150 mm,5μm),以磷酸盐缓冲液(pH6.8)-甲醇(体积比为80∶20)为流动相,检测波长为260 nm,流速为1 mL.min-1,进样量为20μL。结果腺苷和虫草素之间能够达到很好的分离,供试品中其他杂质峰对2个主峰测定无干扰。腺苷和虫草素的线性均为5～150 mg.L-1,r=0.999 7(n=6),平均回收率分别为99.6%和100.3%,RSD分别1.6%和3.0%(n=6)。腺苷和虫草素对照品溶液在24 h内稳定。结论本法一次提取进样可同时分别测定冬虫夏草和蛹虫草子实体中两种有效成分的含量,分析结果准确可靠,可作为该2种种药材质量控制的分析方法。
Objective To establish a method for the determination of adenosine and cordycepin in Cordyceps sinensis and Cordyceps militaris. Methods The samples were extracted by water and the two components were determined by HPLC-UV simultaneously. The chromatographic conditions were as follows: Eclipse-C18 column (4.6 mm × 150 mm, 5 μm) with phosphate buffer (pH 6.8) -methanol (80:20 by volume) 1 mL.min-1, injection volume of 20μL. Results Adenosine and cordycepin can be well separated, for the other impurities in the test peak on the two main peak determination without interference. The linear regression curves of adenosine and cordycepin were 5-150 mg.L-1, r = 0.999 7 (n = 6) and the average recoveries were 99.6% and 100.3%, respectively, with RSDs of 1.6% and 3.0% ). Adenosine and Cordycepin reference solution was stable within 24 h. Conclusions This method can be used for the simultaneous determination of two active ingredients in Cordyceps sinensis and Cordyceps militaris respectively by one extraction method. The results are accurate and reliable, which can be used as the analytical method for the quality control of the two medicinal materials.