目的：研究并介绍一种新的离体气管平滑肌舒缩性检测法——压力检测法。方法：采用测压法检测六只完整离体豚鼠气管平滑肌舒缩功能。结果：当注入舒张药异丙肾上腺素时，能记录到舒张曲线；当注入收缩药乙酰胆碱时，能记录到收缩曲线。结论：实验检测证明本方法具有操作简便、灵敏度高、数据准确、曲线清晰、不破坏离体气管结构等优点，是豚鼠离体气管平滑肌功能检测的较佳方法。 OBJECTIVE: To study and introduce a new method for measuring the contractility of tracheal smooth muscle - pressure test. Methods: The systolic and diastolic function of six intact guinea pig tracheal smooth muscles were measured by manometry. RESULTS: Diastolic curves were recorded when the diastolic isoproterenol was infused; contraction curves were recorded when contractile acetylcholine was infused. Conclusion: The experimental results show that the method has the advantages of simple operation, high sensitivity, accurate data, clear curve and no damage to the tracheal structure. It is a better method to detect the function of isolated tracheal smooth muscle in guinea pigs.