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为了明确p93在心血管系统中的生物学功能及在信号传导通路中的作用 ,采用酵母双杂交技术 ,选择 p93N端第 10 1～ 372位氨基酸构建诱饵质粒 ,筛选成人心脏cDNA文库 ,寻找与之相互作用的蛋白质 ,结果得到蛋白peroxiredoxin 3(PRX 3) .采用体外结合实验和免疫共沉淀等方法 ,证实了原核和真核表达的 p93确实可与PRX3相互作用 ,通过双重荧光共定位 ,为二者的相互作用提供了定位依据 .研究提示p93可能参与心肌细胞中与PRX3有关的各种生理病理过程 ,如生长、分化、发育、凋亡、氧应激等
In order to clarify the biological function of p93 in cardiovascular system and its role in signal transduction pathway, yeast two-hybrid technique was used to construct bait plasmids from amino acids 101 to 372 of p93N and to screen adult cardiac cDNA libraries for mutual interaction (PRX 3). The results of in vitro binding assay and co-immunoprecipitation demonstrated that prokaryotic and eukaryotic expression of p93 did indeed interact with PRX3, The study suggests that p93 may be involved in a variety of physiological and pathological processes related to PRX3 in cardiomyocytes, such as growth, differentiation, development, apoptosis, oxygen stress, etc.