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目的探讨辛伐他汀对蛛网膜下腔出血大鼠海马肿瘤坏死因子-α(TNF-α)的影响。方法将SD大鼠分为对照组、模型组和治疗组,采用非开颅血管内穿线法制备大鼠SAH模型,治疗组给予辛伐他汀。采用免疫组织化学和免疫印迹方法检测大鼠海马TNF-α表达。结果模型组神经功能缺损明显,海马TNF-α表达明显高于对照组(P<0.05)。使用辛伐他汀后,TNF-α表达降低(P<0.05)。结论 TNF-α参与了蛛网膜下腔出血发病过程,辛伐他汀可能通过影响TNF-α途径发挥治疗蛛网膜下腔出血脑血管痉挛作用。
Objective To investigate the effect of simvastatin on tumor necrosis factor-α (TNF-α) in the hippocampus of rats after subarachnoid hemorrhage. Methods The SD rats were divided into control group, model group and treatment group. SAH model was established by intranasal non-craniotomy. Simvastatin was given to the treatment group. Immunohistochemistry and Western blotting were used to detect the expression of TNF-α in hippocampus of rats. Results The neurological deficit in the model group was obvious. The expression of TNF-α in hippocampus was significantly higher than that in the control group (P <0.05). After using simvastatin, TNF-α expression decreased (P <0.05). Conclusions TNF-α is involved in the pathogenesis of subarachnoid hemorrhage. Simvastatin may play a role in the treatment of cerebral vasospasm due to subarachnoid hemorrhage by affecting TNF-α pathway.