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目的研究β-榄香烯对人宫颈Hela细胞凋亡相关蛋白caspase-3、caspase-9表达和PI-3K/Akt信号通路的影响。方法实验分为对照组和β-榄香烯处理组,应用AnnexinV-FITC凋亡检测试剂盒及流式细胞仪检测细胞凋亡率,并采用免疫荧光、Western Blot方法检测Akt、p-Akt、天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3),天冬氨酸特异性半胱氨酸蛋白酶9(caspase-9)、凋亡诱导因子(AIF)、细胞色素C(Cyto c)蛋白表达。结果β-榄香烯以剂量和时间依赖方式抑制宫颈癌细胞增殖和细胞活性。与对照组相比,β-榄香烯处理组凋亡细胞数量和相关凋亡蛋白(caspase-3、caspase-9、AIF、Cyto c)表达水平升高,β-榄香烯作用组p-Akt的表达水平明显升高。结论β-榄香烯抑制宫颈癌细胞增殖和诱导细胞凋亡可能与上调凋亡相关蛋白表达和PI-3K/Akt信号通路相关。
Objective To study the effects of β-elemene on the expression of caspase-3, caspase-9 and PI-3K / Akt in human cervical Hela cells. Methods The experiment was divided into control group and β-elemene treatment group, apoptosis rate was detected by Annexin V-FITC apoptosis detection kit and flow cytometry, and Akt, p-Akt, Caspase-3, caspase-9, AIF, Cyto c, )Protein. Results β-elemene inhibited cervical cancer cell proliferation and cell activity in a dose-and time-dependent manner. Compared with the control group, the expression of apoptotic cells and related apoptotic proteins (caspase-3, caspase-9, AIF and Cyto c) increased in β-elemene treated group, Akt expression was significantly increased. Conclusion The inhibitory effect of β-elemene on the proliferation and apoptosis of cervical cancer cells may be related to the up-regulation of apoptosis-related protein expression and PI-3K / Akt signaling pathway.