论文部分内容阅读
目的通过观察细菌脂多糖(LPS)对海马CA2、3区nNOS和c-Fos表达的影响,探讨海马CA2、3区的免疫调节作用及其与NO和c-Fos蛋白的相关性。方法实验组SD大鼠腹腔注射LPS,对照组注射生理盐水,2.5 h后,采用RT-PCR方法检测大鼠海马CA2、3区nNOS mRNA、原位杂交技术检测该区c-Fos mR-NA,免疫组化方法分别检测该区nNOS和c-Fos的表达变化。结果对照组和腹腔注射LPS组大鼠海马CA2、3区的nNOS,c-Fos及其mRNA的OD值如下:nNOS mRNA(0.283±0.09,0.476±0.03),nNOS(0.653±0.97,1.155±0.12),c-Fos mRNA(1.031±0.99,1.326±0.91),c-Fos(0.426±0.16,0.830±0.14);LPS使大鼠海马CA2、3区nNOS和c-Fos mRNA及表达产物含量均上调。结论海马CA2、3区在机体的免疫应激反应中起重要调节作用,NO和c-Fos蛋白可能是该调节过程中的重要信使分子。
OBJECTIVE: To investigate the effects of lipopolysaccharide (LPS) on the expression of nNOS and c-Fos in hippocampal CA2 and c-Fos regions of hippocampus, and to investigate the immunoregulatory effects of NO and c-Fos protein in hippocampal CA2,3 area. Methods Rats in experimental group were injected intraperitoneally with LPS and control group with normal saline. After 2.5 h, the nNOS mRNA in hippocampus CA2 and 3 were detected by RT-PCR. The c-Fos mR-NA was detected by in situ hybridization. Immunohistochemistry was used to detect the expression of nNOS and c-Fos in this area. Results The OD value of nNOS, c-Fos and their mRNA in hippocampal CA2 area 3 of control group and intraperitoneal injection of LPS group were as follows: nNOS mRNA (0.283 ± 0.09,0.476 ± 0.03), nNOS (0.653 ± 0.97,1.155 ± 0.12 ), c-Fos mRNA (1.031 ± 0.99,1.326 ± 0.91) and c-Fos (0.426 ± 0.16,0.830 ± 0.14). LPS upregulated the expression of nNOS and c-Fos mRNA and protein . Conclusion The hippocampal CA2 and 3 regions play an important role in the immune response to stress. NO and c-Fos may be important messenger molecules in this process.