通过对棉花枯萎镰刀菌生理型Ⅱ、Ⅲ号原生质体的分离、纯化和再生条件的研究,摸清了同龄小型分生孢子在25℃下振荡培养30—40小时的菌丝,在0.6MNacl为稳定剂、4%的混合酶、39℃下酶解3—4小时能得到较高数量的原生质体。在高渗培养基上培养12小时即出现再生,2—3天后再生率达到66—83%。这一工作为进一步研究致病力变异奠定了基础。 Through the study on the isolation, purification and regeneration conditions of the physiological protoplasts of Fusarium solani Ⅱ and Ⅲ of cotton, the conidia of the same age were found to shake for 30-40 hours at 25 ℃, Stabilizer, 4% mixed enzyme, 39 ℃ digestion for 3-4 hours to get a higher number of protoplasts. Regeneration occurs after 12 hours of culture on hypertonic medium, and the regeneration rate reaches 66-83% after 2-3 days. This work laid the foundation for further study of pathogenicity variation.