目的 :建立RP HPLC法测定尼莫地平血浆浓度 ,探讨尼莫地平血药浓度测定的临床意义。方法 :采用HypersillC8色谱柱 (15 0mm× 4 .6mm ,5 μm ) ,流动相为甲醇 - 0 .0 1mol·L-1磷酸二氢钾溶液 (6 1∶39,pH =4 .5 ) ,流速为 1.0ml·min-1,检测波长为 2 38nm ,柱温为 4 5℃。结果 :尼莫地平在 5 .7～ 10 1.7ng·ml-1范围内呈良好线性关系 ,回归方程为Y =4 .76 5 9× 10 -3 X +0 .0 0 96 ,r =0 .9972。平均相对回收率为 98.2 % ,日内及日间RSD均小于 10 %。患者服用不同厂家制剂后的血药浓度具有极显著差异 (P <0 .0 1) ;肝功能正常及不正常患者服用相同厂家制剂后的血药浓度也具有极显著差异 (P <0 .0 1)。结论 :本法灵敏、准确、专属性强 ,适于尼莫地平血浓度测定 OBJECTIVE: To establish a RP-HPLC method for the determination of nimodipine plasma concentration and to explore the clinical significance of nimodipine plasma concentration determination. Methods: Hypersill C8 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol-0.1mol·L-1 potassium dihydrogen phosphate solution (6 1:39, pH = 4.5) 1.0ml · min-1, the detection wavelength was 2 38nm, the column temperature was 45 ℃. Results: Nimodipine showed a good linear relationship in the range of 5.7-7.10ng / ml and the regression equation was Y = 4.76 5 9 × 10 -3 X +0. 0 0 96, r = 0. 9972. The average relative recovery was 98.2% with intraday and daytime RSDs less than 10%. Patients taking different manufacturers after the preparation of the plasma concentration has a very significant difference (P <0.01); normal and abnormal liver function in patients with the same manufacturers after the preparation of the plasma concentration has a very significant difference (P <0 .0 1). Conclusion: This method is sensitive, accurate and specific, suitable for the determination of nimodipine blood concentration