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目的 通过静脉注入抗ＣＤ４ 、ＣＤ８ 免疫毒素及供体可溶性抗原诱导胰岛移植物免疫耐受。方法 供、受体分别为Ｗｉｓｔａｒ 大鼠和ＳＤ大鼠， 移植前１４ 天、７ 天分别将免疫毒素各２００ μｇ， 供体可溶性抗原５００ μｇ 经静脉注入受体， 然后将供体５００ 个胰岛移植于受体（ 糖尿病大鼠）左侧肾包膜下。结果 用免疫毒素及供体可溶性抗原联合处理组胰岛移植物存活时间显著延长（ Ｐ＜ ０ ．０１） ， 而单独应用抗ＣＤ４ 、ＣＤ８ 免疫毒素或供体可溶性抗原组仅能获得胰岛移植物存活时间轻度延长。结论 抗ＣＤ４、ＣＤ８ 免疫毒素及供体可溶性抗原联合应用可以诱导供体特异性免疫耐受
Objective To induce islet graft immune tolerance by intravenous injection of anti-CD4, CD8 immunotoxin and donor soluble antigen. Methods Wistar rats and SD rats were divided into two groups: 200 μg immunotoxins and 500 μg donor soluble antigens were injected into the recipients on the 14th day and the 7th day before transplantation. Then, 500 donor islets were transplanted In the recipient (diabetic rats) the left renal capsule. Results The survival time of islet grafts with immunotoxin and donor soluble antigen was significantly prolonged (P <0.01), whereas the treatment with anti-CD4, CD8 immunotoxin or donor soluble antigen alone could only obtain islet graft survival time Slightly extended. Conclusion Anti-CD4, CD8 immunotoxin and donor soluble antigen can induce donor-specific immune tolerance