论文部分内容阅读
建立离子液体1-己基-3-甲基咪唑六氟磷酸盐([HMIM]PF_6)/甲醇溶液为萃取剂超声萃取,结合反相高效液相色谱同时测定山高粱中槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚4种黄酮成分的方法。采用Purospher star RP-C_(18)色谱柱(4.6 mm×250 mm,5μm)进行分离,以乙腈-甲醇-0.4%磷酸水溶液梯度洗脱,流速0.7 mL·min~(-1),紫外检测波长360 nm,柱温室温。在优化条件下,槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚分别在2.54×10~(-2)~2.54,2.50×10~(-2)~2.50,1.54×10~(-3)~0.154,1.49×10~(-3)~0.149μg线性关系良好,平均加样回收率分别为101.1%,98.90%,101.0%,101.6%。该方法绿色环保、简单快速,结果准确可靠,为山高粱中槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-β-D-葡萄糖苷、槲皮素和山柰酚的分析提供了有效的方法。
The ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM] PF_6) / methanol was used as extraction solvent for ultrasonic extraction. The simultaneous determination of quercetin -3-O in mountain sorghum by reversed-phase high performance liquid chromatography -β-D-glucoside, kaempferol-3-β-D-glucoside, quercetin and kaempferol. The sample was separated on a Purospher star RP-C 18 column (4.6 mm × 250 mm, 5 μm) and eluted with a gradient of acetonitrile-methanol-0.4% phosphoric acid solution at a flow rate of 0.7 mL · min -1. UV detection wavelength 360 nm, column temperature room temperature. Under the optimal conditions, the contents of quercetin -3-O-β-D-glucoside, kaempferol-3-β-D-glucoside, quercetin and kaempferol were respectively in the range of 2.54 × 10 -2 ~ 2.54,2.50 × 10 ~ (-2) ~ 2.50,1.54 × 10 ~ (-3) ~ 0.154,1.49 × 10 ~ (-3) ~ 0.149μg, the average recoveries were 101.1% 98.90%, 101.0%, 101.6%. The method is green, simple and fast, and the method is accurate and reliable. The method is characterized by the following steps: quercetin -3-O-β-D-glucoside, kaempferol-3-β-D-glucoside, quercetin, Analysis of phenol provides an effective method.