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为优化甘蔗试管苗瓶外生根的环境条件,提高成活率,降低生产成本,进行了外界环境因素对甘蔗无根试管苗瓶外生根及生长的影响研究。结果表明,高肥力基质(如商品基质)处理的无根试管苗成活率低,但成活植株生长快,植株高;而低肥力基质(如新鲜河沙土)有利于无根试管苗生根成活,但植株生长慢;适合甘蔗无根试管苗移栽的基质包括新鲜河沙土、田园土和河沙与田园土的混合物。温度、光照和湿度是甘蔗试管苗瓶外生根维持高存活率的关键外部因素,利用遮阳网和盖膜等措施调节温度、湿度和光照可使试管苗的生长环境逐渐过渡到自然环境条件。试管苗瓶外生根技术有利于简化传统甘蔗试管苗生产,降低生产成本,促进甘蔗健康种苗的推广应用。
In order to optimize the environmental conditions for rooting of sugarcane in vitro, increase the survival rate and reduce the production cost, the effects of external environmental factors on rooting and growth of non-rooted tube seedlings of sugarcane were studied. The results showed that the survival rate of rootless test-tube seedlings treated with high-fertility substrate (such as commercial substrate) was low, but the surviving plants grew fast and the plant height. However, the low-fertility substrate (such as fresh river sand soil) Plant growth is slow; suitable for sugar cane rootless test tube seedlings transplanting matrix, including fresh river sand, pastoral soil and river sand and pastoral soil mixture. Temperature, light and humidity are the key external factors to maintain high survival rate of sugar cane in vitro. The use of shading net and cover film to adjust the temperature, humidity and light can make the growth of the test tube plant gradually transition to the natural environment. Test-tube seedling rooting technology outside the bottle is conducive to simplifying the traditional sugar cane plantlet production, reduce production costs and promote the promotion and application of healthy seedlings of sugar cane.