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目的建立大鼠复发性口腔溃疡(ROU)动物模型,探讨T淋巴细胞亚群与ROU发病机制的关系,观察绞股蓝皂甙对大鼠ROU模型的免疫调节作用。方法用免疫方法建立大鼠ROU动物模型并观察大鼠的口腔表现,将实验大鼠随机分为4组:正常对照组、模型组、左旋咪唑组和绞股蓝皂甙组。分别于成模前后及给药后大鼠眼眶采血,用流式细胞仪检测大鼠外周血T淋巴细胞亚群CD4+、CD8+细胞数量及比值。结果①实验大鼠于第3次注射1周后开始出现口腔溃疡,模型组大鼠外周血CD4+及CD4+/CD8+较正常对照组显著降低(P<0.05),CD8+改变无统计学意义(P>0.05)。②实验大鼠给药后,绞股蓝皂甙组和左旋咪唑组外周血CD4+、CD4+/CD8+均有回升,较模型组有显著性差异(P<0.05),两组之间比较没有显著性差异(P>0.05)。结论 ROU的发病与T淋巴细胞亚群的失衡有关。绞股蓝皂甙对大鼠ROU模型有一定的免疫调节作用,与左旋咪唑无明显差异。
Objective To establish an animal model of recurrent oral ulcer (ROU) in rats and explore the relationship between T lymphocyte subsets and the pathogenesis of ROU and to observe the immunoregulatory effect of Gypenosides on ROU model in rats. Methods The animal model of ROU was established by immunization and the oral performance of rats was observed. The rats were randomly divided into 4 groups: normal control group, model group, levamisole group and gypenoside group. Blood samples were taken from the orbit of rats before and after injection into the model and the number of CD4 + and CD8 + cells in the peripheral blood of the rats were determined by flow cytometry. Results ① The oral ulcers began to appear in the experimental rats one week after the third injection. The levels of CD4 + and CD4 + / CD8 + in the peripheral blood of the model group were significantly lower than those of the normal control group (P <0.05), but there was no significant difference in the CD8 + 0.05). (2) The levels of CD4 +, CD4 + / CD8 + in peripheral blood of Gypenoside and Levamisole groups both recovered after the administration of experimental rats, which was significantly different from that of the model group (P <0.05). There was no significant difference between the two groups > 0.05). Conclusion The incidence of ROU is related to the imbalance of T lymphocyte subsets. Gypenosides on rat ROU model has some immunomodulatory effect, and levamisole no significant difference.