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研究丹参水提物在大鼠肠内的吸收特征,采用大鼠肠外翻模型,选择丹参水提物中具有代表性的原儿茶醛(protocatechuic aldehyde,PAL)、迷迭香酸(rosmarinic acid,RA)和丹酚酸B(salvianolic acid B,SAB)3种成分为考察对象,建立其超高效液相(UPLC)测定方法并测量其累积吸收量,阐明各成分在不同肠段的吸收特征。实验结果显示,RA,SAB在回肠内为被动吸收,符合一级速度过程;RA,SAB在空肠内低、中剂量时为零级速度过程,高剂量时为一级速度过程,是一种主、被动同时存在的吸收方式;PAL在空肠、回肠内均为被动吸收。小肠不同部位的吸收实验表明,空肠可能为RA和SAB的主要吸收部位,回肠可能为PAL的主要吸收部位。丹参水提物中PAL,RA,SAB 3种成分在小肠均有吸收,但各成分的吸收速率及其机制不尽相同,提示小肠对丹参水提物各成分的吸收具有选择性,并不是简单地半透膜渗透过程。
To study the absorption characteristics of aqueous extract from Radix Salviae Miltiorrhizae in rat intestine. The model of intestinal eversion was used to select the representative protocatechuic aldehyde (PAL) and rosmarinic acid , RA) and salvianolic acid B (SAB) were investigated in this study. The UPLC method was established and the cumulative absorption was measured. The absorption characteristics of each component in different intestinal segments were elucidated. . The experimental results show that RA and SAB are passive absorption in the ileum, which is consistent with the first-order speed process. RA and SAB are the zero-order speed process in low and middle jejunum, and the first-order speed process in high dose. , Passive simultaneous absorption mode; PAL in the jejunum, ileum are passive absorption. Absorption experiments on different parts of the small intestine showed that the jejunum may be the main absorption site for RA and SAB, and the ileum may be the main absorption site for PAL. Salvia miltiorrhiza water extracts in PAL, RA, SAB three kinds of ingredients are absorbed in the small intestine, but the various components of the absorption rate and mechanism are not the same, suggesting that the small intestine Salvia aqueous extract of each component of the absorption is not simple To the semipermeable membrane infiltration process.