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目的 :采用高效液相色谱法同时测定复方氨酚烷胺胶囊中对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量。方法 :使用C18柱 (Eclipse4 .6mm× 15 0mm) ,柱温 30℃ ,流动相 :乙腈 pH3.0缓冲液 (15 :85 ) ;检测波长 2 14nm ;流速 1ml/min。 结果 :采用高效液相色谱法测定对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量 ,线性范围分别为 2 0 0～ 12 0 0 ,12 .2 8～ 73.6 8,1.0 5～ 6 .30 μg/ml,r =0 .9999;平均回收率分别为 99.8% (RSD =0 .6 % )、10 0 .0 % (RSD =0 .3% )、98.4 % (RSD =0 .9% )。结论 :本法简便快速准确 ,用高效液相色谱法同时测定复方氨酚烷胺胶囊中三个组分的含量 ,能更好地控制产品质量
OBJECTIVE: To simultaneously determine acetaminophen, caffeine and chlorpheniramine maleate in compound paracetamol capsules by high performance liquid chromatography. Methods: C18 column (Eclipse4. 6mm × 150 mm) was used. The column temperature was 30 ℃. The mobile phase was acetonitrile pH 3.0 buffer (15:85). The detection wavelength was 214 nm. The flow rate was 1 ml / min. Results: The contents of acetaminophen, caffeine and chlorpheniramine maleate were determined by high performance liquid chromatography with linear ranges of 200 ~ 1200, 12.28 ~ 73.6 8, 1.05 ~ The average recoveries were 99.8% (RSD = 0.6%), 100.0% (RSD = 0.3%) and 98.4% (RSD = 0. 9% ). Conclusion: The method is simple, rapid and accurate, and the content of three components in compound paracetamol capsules can be determined simultaneously by high performance liquid chromatography to better control the product quality